LOCUS       sequence-83860-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS 
            (st7) gfp::Asp terminator + ColE1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1278
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(108..194)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(303..326)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(501..520)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tttacgcatt ttttttcctc cttacccggg agtatcttgt tatccgctca caatgtcaat
       61 gttatccgct cacatttatg gatccgcgcc taggtctagg gcggcggatt tgtcctactc
      121 aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc
      181 tttcgtttta tttgatgcct ctagcacgcg taccatgggc ccttacttgt acagctcgtc
      241 catgccgccg gttgagtggc ggccctcggc gcgctcgtac tgttccacga tggtgtagtc
      301 ctcgttgtgg gaggtgatgt ccagcttgat gttgacgttg taggcgccgg gaagctgcac
      361 gggcttcttg gccttgtagg tggtcttgac ctcggcgtcg tagtggccgc cgtccttcag
      421 cttcagcctc tgcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcgga
      481 ggaggcctcc cagcccattg tcttcttctg cattacgggg ccgtcggagg ggaagttggt
      541 gccgcgcagc ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac
      601 ggtcaccacg ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa
      661 ggacagcttc aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta
      721 catgaactga ggggacagga tgtcccaggc gaagggcagg gggccgccct tggtcacctt
      781 cag
//
