LOCUS       sequence-83862-e         679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1033
FEATURES             Location/Qualifiers
     source          1..679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    216..232
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        290..394
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(613..632)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 catgctcgcg gtacctttaa tcaggttgtt caggttagtg acataagtct gatggtgctt
       61 gccgtagtga tactcgatgg tttccgcaga aatgtgcggt gccagagcat ctttagcata
      121 tggtagtgca ggtaattcga atgacattgc tactctcctt tattattaat ttgcatacgt
      181 acaatagcct tattgtgcgt atggtcgaca gtatcttgtt atccgctcac aatgtcaatg
      241 ttatccgctc acatttatga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      301 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      361 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      421 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      481 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      541 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      601 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      661 cggtcgccgc atacactat
//
