LOCUS       sequence-83863-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1035
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    224..240
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        298..402
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(621..640)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctttacgca tgctcgcggt acctttaatc aggttgttca ggttagtgac ataagtctga
       61 tggtgcttgc cgtagtgata ctcgatggtt tccgcagaaa tgtgcggtgc cagagcatct
      121 ttagcatatg gtagtgcagg taattcgaat gacattgcta ctctccttta ttattaattt
      181 gcatacgtac aatagcctta ttgtgcgtat ggtcgacagt atcttgttat ccgctcacaa
      241 tgtcaatgtt atccgctcac atttatgacg tcaggtggca cttttcgggg aaatgtgcgc
      301 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      361 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
      421 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
      481 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
      541 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
      601 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
      661 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
      721 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
      781 atgagtgata acactgcggc caacttact
//
