LOCUS       sequence-83865-e         672 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLlac0-1:OxyS-mCherry, pLtetO-1:fhlA::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 100
FEATURES             Location/Qualifiers
     source          1..672
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    224..242
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    249..267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(288..306)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        374..478
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tacgcatgct ctcacacagc gaggccagat cgggctgctg caatagtgtc cgagtgatgt
       61 cgaacaaccc ttgttgtccg agatcactca tcggtgtata tgacattttg ttcttccagg
      121 aaagcaccgc aaagcggtgc aaaaggtcat tgactaacta taggagattt agaattttat
      181 tagcatatac gcggaagcgg ttcggcgtgt ggtgtcgaca gtatctctat cactgatagg
      241 gatgtcaatc tctatcactg atagggactc gaggtgaaga cgaaagggcc tcgtgatacg
      301 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
      361 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
      421 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
      481 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
      541 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
      601 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
      661 agaacgtttt cc
//
