LOCUS       sequence-83866-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLlac0-1:RhyB-mCherry, pLtetO-1:RBS (st7) gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1011
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    62..80
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(101..119)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        187..291
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(510..529)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctttacgca ttttttttcc tccttagtcg acagtctctc tatcactgat agggatgtca
       61 atctctatca ctgataggga ctcgaggtga agacgaaagg gcctcgtgat acgcctattt
      121 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
      181 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      241 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      301 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      361 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      421 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      481 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      541 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      601 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      661 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      721 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      781 gaaccggagc
//
