LOCUS       sequence-83870-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:DsrA, pCon:OxyS, pLlacO-1:rpoS::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1039
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    204..220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        278..382
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(601..620)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tttacgcatg ctatcatgaa ctttcagcgt attctgactc ataaggtggc tcctacccgt
       61 gatcccttga cggaacattc aagcaaaagc ctggttccgc cgatttatcg ctgcggcaaa
      121 taacgcagcg ggtttacgga tttccccttg taacgaattt caaaatgcaa gcgtgttgaa
      181 ctggttccgg tgtcgacagt atcttgttat ccgctcacaa tgtcaatgtt atccgctcac
      241 atttatgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      301 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      361 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      421 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      481 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      541 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      601 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      661 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      721 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      781 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttt
//
