LOCUS       sequence-83872-e         782 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:DsrA, pLlacO-1:sodB::gfp
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1020
FEATURES             Location/Qualifiers
     source          1..782
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    225..241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        299..403
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(622..641)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcctttacgc atgctcgcgg tacctttaat caggttgttc aggttagtga cataagtctg
       61 atggtgcttg ccgtagtgat actcgatggt ttccgcagaa atgtgcggtg ccagagcatc
      121 tttagcatat ggtagtgcag gtaattcgaa tgacattgct actctccttt attattaatt
      181 tgcatacgta caatagcctt attgtgcgta tggtcgacag tatcttgtta tccgctcaca
      241 atgtcaatgt tatccgctca catttatgac gtcaggtggc acttttcggg gaaatgtgcg
      301 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
      361 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
      421 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
      481 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
      541 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
      601 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      661 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      721 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      781 ca
//
