LOCUS       sequence-83873-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:mCherry, PLlacO-1:gfp (st7)
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Hussein R, Lim HN
  TITLE     Direct comparison of small RNA and transcription factor signaling.
  JOURNAL   Nucleic Acids Res. 2012 May 22.
  PUBMED    22618873
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2012 May 22."
COMMENT     Sequence Label: pHL 1023
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        113..217
                     /gene="bla"
                     /label=AmpR promoter
     CDS             402..780
                     /label=ISS
                     /note="immunostimulatory sequence from the AmpR gene; 
                     contains unmethylated CpG dinucleotides in the context of 
                     5'-AACGTT-3' (Sato et al., 1996)"
     primer_bind     complement(436..455)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cctttacgca ttttttttcc tccttagtcg acagtatctt gttatccgct cacaatgtca
       61 atgttatccg ctcacattta tgacgtcagg tggcactttt cggggaaatg tgcgcggaac
      121 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
      181 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
      241 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
      301 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
      361 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
      421 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
      481 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
      541 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
      601 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
      661 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
      721 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
      781 gcgcaaacta ttaactggcg aactacttac tctagcttcc
//
