LOCUS       sequence-83876-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNAi vector for Sirt7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Barber MF, Michishita-Kioi E, Xi Y, Tasselli L, Kioi M, Moqtaderi Z,
            Tennen RI, Paredes S, Young NL, Chen K, Struhl K, Garcia BA, Gozani 
            O, Li W, Chua KF
  TITLE     SIRT7 links H3K18 deacetylation to maintenance of oncogenic 
            transformation.
  JOURNAL   Nature. 2012 Jul 5;487(7405):114-8. doi: 10.1038/nature11043.
  PUBMED    22722849
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-07-5- 5"; volume: "487"; issue: "7405"; pages:
            "114-8"
COMMENT     Sequence Label: pSUPERretro-Sirt7 shRNA1
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     36..52
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(125..339)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(185..204)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(385..408)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ctccttctct aggcgccgga attagatcga tctctcgagg tcgacggtat cgataagctt
       61 aaaaacacct ttctgtgaga acggaatctc ttgaattccg ttctcacaga aaggtggggg
      121 atctgtggtc tcatacagaa cttataagat tcccaaatcc aaagacattt cacgtttatg
      181 gtgatttccc agaacacata gcgacatgca aatattgcag ggcgccactc ccctgtccct
      241 cacagccatc ttcctgccag ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg
      301 cccgcgattc cttggagcgg gttgatgacg tcagcgttcg aattctaccg ggtaggggag
      361 gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg cacttggcgc
      421 tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg ccaaccggct
      481 ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag gaagttcccc
      541 cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg tctcactagt
      601 ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg gggcagcggc
      661 caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg tgggtccggg
      721 ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
//
