LOCUS       sequence-83877-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNAi vector for Sirt7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Barber MF, Michishita-Kioi E, Xi Y, Tasselli L, Kioi M, Moqtaderi Z,
            Tennen RI, Paredes S, Young NL, Chen K, Struhl K, Garcia BA, Gozani 
            O, Li W, Chua KF
  TITLE     SIRT7 links H3K18 deacetylation to maintenance of oncogenic 
            transformation.
  JOURNAL   Nature. 2012 Jul 5;487(7405):114-8. doi: 10.1038/nature11043.
  PUBMED    22722849
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2012-07-5- 5"; volume: "487"; issue: "7405"; pages:
            "114-8"
COMMENT     Sequence Label: pSUPERretro-Sirt7 shRNA2
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..56
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     41..57
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(130..344)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(190..209)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(390..413)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattag atcgatctct cgaggtcgac ggtatcgata
       61 agcttaaaaa tagccatttg tccttgagga atctcttgaa ttcctcaagg acaaatggct
      121 aggggatctg tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt
      181 ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc cactcccctg
      241 tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca
      301 ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc taccgggtag
      361 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      421 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      481 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      541 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
      601 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
      661 gcggccaata gcagctttgc tccttcgctt tctggg
//
