LOCUS       sequence-83879-e         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNAi vector for Sirt6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Tennen RI, Bua DJ, Wright WE, Chua KF
  TITLE     SIRT6 is required for maintenance of telomere position effect in 
            human cells.
  JOURNAL   Nat Commun. 2011 Aug 16;2:433. doi: 10.1038/ncomms1443.
  PUBMED    21847107
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2011-08-16"; volume: "2:433. doi"; pages: " 
            10.1038/ncomms1443"
COMMENT     Sequence Label: pSUPERretro-Sirt6 shRNA2
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..56
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     41..57
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(130..344)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(190..209)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(390..413)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 cctcactcct tctctaggcg ccggaattag atcgatctct cgaggtcgac ggtatcgata
       61 agcttaaaaa aagaatgtgc caagtgtaag atctcttgaa tcttacactt ggcacattct
      121 tggggatctg tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt
      181 ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc cactcccctg
      241 tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca
      301 ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc taccgggtag
      361 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
      421 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
      481 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
      541 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
      601 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
      661 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
      721 ccggggg
//
