LOCUS       sequence-83903-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Luo Y, Liu C, Cerbini T, San H, Lin Y, Chen G, Rao MS, Zou J
  TITLE     Stable Enhanced Green Fluorescent Protein Expression After 
            Differentiation and Transplantation of Reporter Human Induced 
            Pluripotent Stem Cells Generated by AAVS1 Transcription 
            Activator-Like Effector Nucleases.
  JOURNAL   Stem Cells Transl Med. 2014 May 15. pii: sctm.2013-0212.
  PUBMED    24833591
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells 
            Transl Med. 2014 May 15. pii: sctm.2013-0212."
COMMENT     Sequence Label: pZT-AAVS1-R1
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             76..141
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             148..168
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatagggag acccaagctg gctagcgttt aaacttaagc tgatccacta gtccagtgtg
       61 gtggaattcg ccatggacta caaagaccat gacggtgatt ataaagatca tgacatcgat
      121 tacaaggatg acgatgacaa gatggccccc aagaagaaga ggaaggtggg catccacggg
      181 gtacctatgg tggacttgag gacactcggt tattcgcaac agcaacagga gaaaatcaag
      241 cctaaggtca ggagcaccgt cgcgcaacac cacgaggcgc ttgtggggca tggcttcact
      301 catgcgcata ttgtcgcgct ttcacagcac cctgcggcgc ttgggacggt ggctgtcaaa
      361 taccaagata tgattgcggc cctgcccgaa gccacgcacg aggcaattgt aggggtcggt
      421 aaacagtggt cgggagcgcg agcacttgag gccttgctga ctgtggcggg tgagcttagg
      481 gggcctccgc tccagctcga caccgggcag ctgctgaaga tcgcgaagag agggggagta
      541 acagcggtag aggcagtgca cgcctggcgc aatgcgctca ccggggcccc cctgaacctg
      601 accccggacc aagtggtggc tatcgccagc aacggtggcg gcaagcaagc gctcgaaacg
      661 gtgcagcggc tgttgccggt gctgtgccag gaccatggcc tgaccccgga ccaagtggtg
      721 gctatcgcca gcaacggtgg cggcaagcaa gcgctcgaaa cggtgcagcg gctgtttgcc
      781 ggtgctgtgc caggaccatg gcctgacccc ggaccaagtg gtggctatcg ccagcaacgg
      841 tggcggcaag caagcgctcg aaacggtgca gcggctgttg ccggtgctgt gccaggacca
      901 tggcctgact ccggaccaag tggtggctat cgcca
//
