LOCUS       sequence-83904-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Elowitz MB, Leibler S
  TITLE     A synthetic oscillatory network of transcriptional regulators.
  JOURNAL   Nature. 2000 Jan 20. 403(6767):335-8.
  PUBMED    10659856
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2000 Jan 20. 403(6767):335-8."
COMMENT     Sequence Label: pZE21-GFPaav
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             53..64
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(74..99,100..105,106..122,123..128,
                     129..147))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    104..122
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    129..147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(168..186)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(419..438)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 agaattggga caactccagt gaaaagttct tctcctttac gcatgcggta cctttctcct
       61 ctttaatgaa ttcggtcagt gcgtcctgct gatgtgctca gtatctctat cactgatagg
      121 gatgtcaatc tctatcactg atagggactc gaggtgaaga cgaaagggcc tcgtgatacg
      181 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcgg aattgccagc
      241 tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc
      301 gccaaggatc tgatggcgca ggggatcaag atctgatcaa gagacaggat gaggatcgtt
      361 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
      421 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
      481 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
      541 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
      601 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
      661 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
      721 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
      781 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
      841 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc
      901 cgacggcga
//
