LOCUS       sequence-83916-e        1144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal His tag fused human codon-optimized Cas9 
            nuclease having C-terminal Cysteine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ramakrishna S, Kwaku Dad AB, Beloor J, Gopalappa R, Lee SK, Kim H
  TITLE     Gene disruption by cell-penetrating peptide-mediated delivery of 
            Cas9 protein and guide RNA.
  JOURNAL   Genome Res. 2014 Apr 2.
  PUBMED    24696462
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2014 Apr 2."
COMMENT     Sequence Label: pET28a/Cas9-Cys
FEATURES             Location/Qualifiers
     source          1..1144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gaccgagggc atgcgcaagc ccgccttcct gagcggcgag cagaagaagg ccatcgtgga
       61 cctgctgttc aagaccaacc gcaaggtgac cgtgaagcag ctgaaggagg actacttcaa
      121 gaagatcgag tgcttcgaca gcgtggagat cagcggcgtg gaggaccgct tcaacgccag
      181 cctgggcacc taccacgacc tgctgaagat catcaaggac aaggacttcc tggacaacga
      241 ggagaacgag gacatcctgg aggacatcgt gctgaccctg accctgttcg aggaccgcga
      301 gatgatcgag gagcgcctga agacctacgc ccacctgttc gacgacaagg tgatgaagca
      361 gctgaagcgc cgccgctaca ccggctgggg ccgcctgagc cgcaagctta tcaacggcat
      421 ccgcgacaag cagagcggca agaccatcct ggacttcctg aagagcgacg gcttcgccaa
      481 ccgcaacttc atgcagctga tccacgacga cagcctgacc ttcaaggagg acatccagaa
      541 ggcccaggtg agcggccagg gcgacagcct gcacgagcac atcgccaacc tggccggcag
      601 ccccgccatc aagaagggca tcctgcagac cgtgaaggtg gtggacgagc tggtgaaggt
      661 gatgggccgc cacaagcccg agaacatcgt gatcgagatg gcccgcgaga accagaccac
      721 ccagaagggc cagaagaaca gccgcgagcg catgaagcgc atcgaggagg gcatcaagga
      781 gctgggcagc cagatcctga aggagcaccc cgtggagaac acccagctgc agaacgagaa
      841 gctgtacctg tactacctgc agaacggccg cgacatgtac gtggaccagg agctggacat
      901 caaccgcctg agcgactacg acgtggacca catcgtgccc cagagcttcc tgaaggacga
      961 cagcatcgac aacaaggtgc tgacccgcag cgacaagaac cgcggcaaga gcgacaacgt
     1021 gcccagcgag gaggtggtga agaagatgaa gaactactgg cgccagctgc tgaacgccaa
     1081 gctgatcacc cagcgcaagt tcgacaacct gaccaaggcc gagcgcggcg gcctgagcga
     1141 gctg
//
