LOCUS       sequence-83919-e        1108 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminal His tag fused human codon-optimized Cas9 
            nuclease having C-terminal Cysteine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ramakrishna S, Kwaku Dad AB, Beloor J, Gopalappa R, Lee SK, Kim H
  TITLE     Gene disruption by cell-penetrating peptide-mediated delivery of 
            Cas9 protein and guide RNA.
  JOURNAL   Genome Res. 2014 Apr 2.
  PUBMED    24696462
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome Res.
            2014 Apr 2."
COMMENT     Sequence Label: pET28a/Cas9-Cys
FEATURES             Location/Qualifiers
     source          1..1108
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..56)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(78..104)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             complement(78..104)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(105..125)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tcagcttcct ttcgggcttt gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg
       61 agtgcggccg cttaacaggc gtagtcgggc acgtcgtagg ggtatacttt tctctttttc
      121 tttggaggtc cggagccgcc gtcgccgccc agctggctca ggtcgatgcg ggtctcgtac
      181 agaccggtga tgctctggtg gatcagggtg gcgtccagca cctccttggt gctggtgtag
      241 cgcttgcggt cgatggtggt gtcgaagtac ttgaaggcgg cgggggcgcc caggttggtc
      301 agggtgaaca ggtggatgat gttctcggcc tgctcgcgga tgggcttgtc gcggtgcttg
      361 ttgtaggcgc tcagcacctt gtccaggttg gcgtcggcca ggatcacgcg cttgctgaac
      421 tcgctgatct gctcgatgat ctcgtccagg tagtgcttgt gctgctccac gaacagctgc
      481 ttctgctcgt tgtcctcggg gctgcccttc agcttctcgt agtggctggc caggtacagg
      541 aagttcacgt acttgctggg cagggccagc tcgttgccct tctgcagctc gccggcgctg
      601 gccagcatgc gcttgcggcc gttctccagc tcgaacaggc tgtacttggg cagcttgatg
      661 atcaggtcct tcttcacctc cttgtagccc ttggcctcca ggaagtcgat ggggttcttc
      721 tcgaagctgc tgcgctccat gatggtgatg cccagcagct ccttcacgct cttcagcttc
      781 ttgctcttgc ccttctccac cttggccacc accagcacgc tgtaggccac ggtggggctg
      841 tcgaagccgc cgtacttctt ggggtcccag tccttcttgc gggcgatcag cttgtcgctg
      901 ttgcgcttgg gcaggatgct ctccttgctg aagccgccgg tctgcacctc ggtcttcttc
      961 acgatgttca cctggggcat gctcagcacc ttgcgcacgg tggcgaagtc gcggcccttg
     1021 tcccacacga tctcgccggt ctcgccgttg gtctcgatca gggggcgctt gcggatctcg
     1081 ccgttggcca gggtgatctc ggtcttga
//
