LOCUS       sequence-83924-e        1063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEntry Tshld-GFP is a product of BP recombination reaction between 
            attB flanked Tshld system-encoded PCR product and pDonor 201 
            plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Lin YH, Pratt MR
  TITLE     A Dual Small-Molecule Rheostat for Precise Control of Protein 
            Concentration in Mammalian Cells.
  JOURNAL   Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006.
  PUBMED    24615791
REFERENCE   2  (bases 1 to 1063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006."
COMMENT     Sequence Label: pEntry Tshld-GFP
FEATURES             Location/Qualifiers
     source          1..1063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(join(117..830,831..833,834..836))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(120..830,831..833,834..836))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(162..183)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     511..530
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     770..791
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(843..869)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcaat gctttcttat aatgccaact ttgtacaaga aagctgggtc tctagattac
      121 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
      181 atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg
      241 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
      301 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc
      361 ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc
      421 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
      481 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
      541 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
      601 atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg
      661 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
      721 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
      781 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatgaat
      841 tccgcgtagt ctggcacgtc gtaggggtac ataccaccgc ggagtctcaa gaccagatgt
      901 aaggtggact ccttctgaat gttgtaatca gacagcgttc taccgtcttc tagctgctta
      961 ccggcaaaga tcaatctttg ttgatctgga gggataccgc tgccgccgcc gctgccgccg
     1021 ccgctgccgc cgccgctgcc gccgccggta ccttccggtt tta
//
