LOCUS       sequence-83926-e        1052 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEntry Tshld-GFP is a product of BP recombination reaction between 
            attB flanked Tshld system-encoded PCR product and pDonor 201 
            plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Lin YH, Pratt MR
  TITLE     A Dual Small-Molecule Rheostat for Precise Control of Protein 
            Concentration in Mammalian Cells.
  JOURNAL   Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006.
  PUBMED    24615791
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006."
COMMENT     Sequence Label: pEntry Tshld-GFP
FEATURES             Location/Qualifiers
     source          1..1052
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..117
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             121..144
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             157..435
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             607..660
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(670..672,673..993)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
ORIGIN
        1 gcatggatct cgggccccaa ataatgattt tattttgact gatagtgacc tgttcgttgc
       61 aacaaattga tgagcaatgc ttttttataa tgccaacttt gtacaaaaaa gcaggctatg
      121 gactacaaag acgatgacga taaaatggct tccagaatcc tctggcatga gatgtggcat
      181 gaaggcctgg aagaggcatc tcgtttgtac tttggggaaa ggaacgtgaa aggcatgttt
      241 gaggtgctgg agcccttgca tgctatgatg gaacggggcc cccagactct gaaggaaaca
      301 tcctttaatc aggcctatgg tcgagattta atggaggccc aagagtggtg caggaagtac
      361 atgaaatcag ggaatgtcaa ggacctcctc caagcctggg acctctatta tcatgtgttc
      421 cgacgaatct caaagggtac cggcggcggc agcggcggcg gcagcggcgg cggcagcggc
      481 ggcggcagca tgcagatctt cgtgaagacc ctgactggta agaccggcac cctggaggtg
      541 gagcccagtg acaccatcga gaatgtcaag gcaaagatcc aagataagga aggcatccct
      601 ggcggcgagg gcaggggctc cctgctgacc tgcggcgacg tggaggagaa ccccggcccc
      661 atgctcgaga tgggagtgca ggtggaaacc atctccccag gagacgggcg caccttcccc
      721 aagcgcggcc agacctgcgt ggtgcactac accgggatgc ttgaagatgg aaagaaattt
      781 gattcctccc gggacagaaa caagcccttt aagtttatgc taggcaagca ggaggtgatc
      841 cgaggctggg aagaaggggt tgcccagatg agtgtgggtc agagagccaa actgactata
      901 tctccagatt atgcctatgg tgccactggg cacccaggca tcatcccacc acatgccact
      961 ctcgtcttcg atgtggagct tctaaaaccg gaaggtaccg gcggcggcag cggcggcggc
     1021 agcggcggcg gcagcggcgg cggcagcggt at
//
