LOCUS       sequence-83939-e        1094 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEntry Tshld-GFP is a product of BP recombination reaction between 
            attB flanked Tshld system-encoded PCR product and pDonor 201 
            plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Lin YH, Pratt MR
  TITLE     A Dual Small-Molecule Rheostat for Precise Control of Protein 
            Concentration in Mammalian Cells.
  JOURNAL   Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006.
  PUBMED    24615791
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006."
COMMENT     Sequence Label: pEntry Tshld-GFP
FEATURES             Location/Qualifiers
     source          1..1094
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(37..63)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(join(247..567,568..570))
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             complement(580..633)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(805..1083)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcaccat gaattccgcg tagtctggca cgtcgtaggg
       61 gtacatacca ccgcggagtc tcaagaccag atgtaaggtg gactccttct gaatgttgta
      121 atcagacagc gttctaccgt cttctagctg cttaccggca aagatcaatc tttgttgatc
      181 tggagggata ccgctgccgc cgccgctgcc gccgccgctg ccgccgccgc tgccgccgcc
      241 ggtaccttcc ggttttagaa gctccacatc gaagacgaga gtggcatgtg gtgggatgat
      301 gcctgggtgc ccagtggcac cataggcata atctggagat atagtcagtt tggctctctg
      361 acccacactc atctgggcaa ccccttcttc ccagcctcgg atcacctcct gcttgcctag
      421 cataaactta aagggcttgt ttctgtcccg ggaggaatca aatttctttc catcttcaag
      481 catcccggtg tagtgcacca cgcaggtctg gccgcgcttg gggaaggtgc gcccgtctcc
      541 tggggagatg gtttccacct gcactcccat ctcgagcatg gggccggggt tctcctccac
      601 gtcgccgcag gtcagcaggg agcccctgcc ctcgccgcca gggatgcctt ccttatcttg
      661 gatctttgcc ttgacattct cgatggtgtc actgggctcc acctccaggg tgccggtctt
      721 accagtcagg gtcttcacga agatctgcat gctgccgccg ccgctgccgc cgccgctgcc
      781 gccgccgctg ccgccgccgg taccctttga gattcgtcgg aacacatgat aatagaggtc
      841 ccaggcttgg aggaggtcct tgacattccc tgatttcatg tacttcctgc accactcttg
      901 ggcctccatt aaatctcgac cataggcctg attaaaggat gtttccttca gagtctgggg
      961 gccccgttcc atcatagcat gcaagggctc cagcacctca aacatgcctt tcacgttcct
     1021 ttccccaaag tacaaacgag atgcctcttc caggccttca tgccacatct catgccagag
     1081 gattctggaa gcca
//
