LOCUS       sequence-83942-e        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination 
            reaction between attB flanked Tshld(F36V) system-encoded PCR product
            and pDonor 201 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Lin YH, Pratt MR
  TITLE     A Dual Small-Molecule Rheostat for Precise Control of Protein 
            Concentration in Mammalian Cells.
  JOURNAL   Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006.
  PUBMED    24615791
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006."
COMMENT     Sequence Label: pEntry Tshld(FKBP F36V)-GFP
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..116
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             120..143
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             156..434
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             606..659
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(669..671,672..992)
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             join(669..671,672..992)
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             join(669..671,672..992)
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
ORIGIN
        1 catggatctc gggccccaaa taatgatttt attttgactg atagtgacct gttcgttgca
       61 acacattgat gagcaatgct tttttataat gccaactttg tacaaaaaag caggctatgg
      121 actacaaaga cgatgacgat aaaatggctt ccagaatcct ctggcatgag atgtggcatg
      181 aaggcctgga agaggcatct cgtttgtact ttggggaaag gaacgtgaaa ggcatgtttg
      241 aggtgctgga gcccttgcat gctatgatgg aacggggccc ccagactctg aaggaaacat
      301 cctttaatca ggcctatggt cgagatttaa tggaggccca agagtggtgc aggaagtaca
      361 tgaaatcagg gaatgtcaag gacctcctcc aagcctggga cctctattat catgtgttcc
      421 gacgaatctc aaagggtacc ggcggcggca gcggcggcgg cagcggcggc ggcagcggcg
      481 gcggcagcat gcagatcttc gtgaagaccc tgactggtaa gaccggcacc ctggaggtgg
      541 agcccagtga caccatcgag aatgtcaagg caaagatcca agataaggaa ggcatccctg
      601 gcggcgaggg caggggctcc ctgctgacct gcggcgacgt ggaggagaac cccggcccca
      661 tgctcgagat gggagtgcag gtggaaacca tctccccagg agacgggcgc accttcccca
      721 agcgcggcca gacctgcgtg gtgcactaca ccgggatgct tgaagatgga aagaaagtgg
      781 attcctcccg ggacagaaac aagcccttta agtttatgct aggcaagcag gaggtgatcc
      841 gaggctggga agaaggggtt gcccagatga gtgtgggtca gagagccaaa ctgactatat
      901 ctccagatta tgcctatggt gccactgggc acccaggcat catcccacca catgccactc
      961 tcgtcttcga tgtggagctt ctaaaaccgg aaggtaccgg cggcggcagc ggcggcggca
     1021 gcggcggcgg cagcggcggc ggca
//
