LOCUS       sequence-83943-e        1104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination 
            reaction between attB flanked Tshld(F36V) system-encoded PCR product
            and pDonor 201 plasmid..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Lin YH, Pratt MR
  TITLE     A Dual Small-Molecule Rheostat for Precise Control of Protein 
            Concentration in Mammalian Cells.
  JOURNAL   Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006.
  PUBMED    24615791
REFERENCE   2  (bases 1 to 1104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2014 Mar 11. doi: 10.1002/cbic.201400006."
COMMENT     Sequence Label: pEntry Tshld(FKBP F36V)-GFP
FEATURES             Location/Qualifiers
     source          1..1104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(39..65)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(join(249..569,570..572))
                     /codon_start=1
                     /product="destabilization domain that can be stabilized by 
                     Shield1 in the ProteoTuner(TM) system"
                     /label=DD
                     /note="L106P mutant of FKBP12"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             complement(join(249..569,570..572))
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein 
                     FKBP12"
                     /label=FKBP-M
                     /note="dimerization can be reversed by ligand binding"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             complement(join(249..569,570..572))
                     /codon_start=1
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=FKBP-V
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKPE"
     CDS             complement(582..635)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(807..1085)
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atgaattccg cgtagtctgg cacgtcgtag
       61 gggtacatac caccgcggag tctcaagacc agatgtaagg tggactcctt ctgaatgttg
      121 taatcagaca gcgttctacc gtcttctagc tgcttaccgg caaagatcaa tctttgttga
      181 tctggaggga taccgctgcc gccgccgctg ccgccgccgc tgccgccgcc gctgccgccg
      241 ccggtacctt ccggttttag aagctccaca tcgaagacga gagtggcatg tggtgggatg
      301 atgcctgggt gcccagtggc accataggca taatctggag atatagtcag tttggctctc
      361 tgacccacac tcatctgggc aaccccttct tcccagcctc ggatcacctc ctgcttgcct
      421 agcataaact taaagggctt gtttctgtcc cgggaggaat ccactttctt tccatcttca
      481 agcatcccgg tgtagtgcac cacgcaggtc tggccgcgct tggggaaggt gcgcccgtct
      541 cctggggaga tggtttccac ctgcactccc atctcgagca tggggccggg gttctcctcc
      601 acgtcgccgc aggtcagcag ggagcccctg ccctcgccgc cagggatgcc ttccttatct
      661 tggatctttg ccttgacatt ctcgatggtg tcactgggct ccacctccag ggtgccggtc
      721 ttaccagtca gggtcttcac gaagatctgc atgctgccgc cgccgctgcc gccgccgctg
      781 ccgccgccgc tgccgccgcc ggtacccttt gagattcgtc ggaacacatg ataatagagg
      841 tcccaggctt ggaggaggtc cttgacattc cctgatttca tgtacttcct gcaccactct
      901 tgggcctcca ttaaatctcg accataggcc tgattaaagg atgtttcctt cagagtctgg
      961 gggccccgtt ccatcatagc atgcaagggc tccagcacct caaacatgcc tttcacgttc
     1021 ctttccccaa agtacaaacg agatgcctct tccaggcctt catgccacat ctcatgccag
     1081 aggattctgg aagccatttt atcg
//
