LOCUS       sequence-83945-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:cstA‡, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1940
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     196..214
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    235..253
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    260..278
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ccaccttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga tcttgatccc
       61 ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca gggcttccca
      121 accttaccag agggcgcccc agctggcaat tccgacgtct aagaaaccat tattatcatg
      181 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcacct cgagtcccta
      241 tcagtgatag agattgacat ccctatcagt gatagagata ctgtcgacaa atgtaacatc
      301 tctatggaca cgcacacgga taacaactat gaacaaatca gggaaatacc tcgtctggac
      361 agtgctctct gtaatgggag catttgctct gggatacatt gctttaaatc gtggggaaca
      421 gatcaacgcg ctgtggattg tggtggcgtc ggtctgtatc tatctgatcg cttaccgttt
      481 ttatgggctg tatatcgcca aaaatgtgct ggcggttgac ccgacgcgta tgacgccagc
      541 ggtgcgccat aacgacgggc tggactatgt gccgacggac aagaaagtgc tgttcggtca
      601 ccattttgcg gccattgccg gagcaggtcc gctggtgggg ccggtactgg cggcgcaaat
      661 gggctacctg ccggggatga tctggctgct cgctggggtg gttctcgccg gtgcggtgca
      721 ggatttcatg gtgctgtttg tttctacgcg ccgtgacggt cgctcgctgg gtgagctggt
      781 caaagaagag atggggccaa ccgccggggt gattgcgctg
//
