LOCUS       sequence-83946-e         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:Agn43RBS:mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1928
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    357..375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    382..400
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(421..439)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(672..691)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
       61 tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcagcatg ctcatgtgat
      121 tccataccag cctgtagcag gtattcagat gtcgtttcat cagcttttcc ttagattgag
      181 ggtgaataaa aaagccggta cccacaaacg tggataccgg caggtaaagg gttacagtgc
      241 cggaggtccg gctgtgggag tttctgaatt gtggttctgt tgttccctga atgtctttct
      301 gtacgtatct gtgcatgtta ctgacaaagg ttattattag cttatcgtcg acagtatctc
      361 tatcactgat agggatgtca atctctatca ctgataggga ctcgaggtga agacgaaagg
      421 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      481 cggaattgcc agctggggcg ccctctggta aggttgggaa gccctgcaaa gtaaactgga
      541 tggctttctt gccgccaagg atctgatggc gcaggggatc aagatctgat caagagacag
      601 gatgaggatc gtttcgcatg attgaacaag gtggattgca cgcaggttct ccggccgctt
      661 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
      721 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
      781 gtgccctgaa tgaact
//
