LOCUS       sequence-83948-e         620 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, 
            PconNoHind:RBS(st3):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1855
FEATURES             Location/Qualifiers
     source          1..620
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(83..100)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      253..339
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      431..458
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 acaaagcagc aaacgacgaa aactacgctt tagtagctta atgctagctt ggctgttttg
       61 gcggatgaga gaagattttc agcctgatac agattaaatc agaacgcaga agcggtctga
      121 taaaacagaa tttgcctggc ggcagtagcg cggtggtccc acctgacccc atgccgaact
      181 cagaagtgaa acgccgtagc gccgatggta gtgtggggtc tccccatgcg agagtaggga
      241 actgccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      301 tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc cgccgggagc ggatttgaac
      361 gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc cgccataaac tgccaggcat
      421 caaattaagc agaaggccat cctgacggat ggcctttttg cgtttctaca aactcttttg
      481 tttatttttc taaatacatt caaatatgta tccgctcagg atcctcgagc accgtcgttg
      541 ttgacatttt tatgcttggc ggttataatg gattccacac acccgggtaa ggaggaaaat
      601 gtctagatta gataaagtaa
//
