LOCUS       sequence-83957-e        1367 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial Expression Vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1367)
  AUTHORS   Hopkins BD, Fine B, Steinbach N, Dendy M, Rapp Z, Shaw J, Pappas K, 
            Yu JS, Hodakoski C, Mense S, Klein J, Pegno S, Sulis ML, Goldstein 
            H, Amendolara B, Lei L, Maurer M, Bruce J, Canoll P, Hibshoosh H, 
            Parsons R
  TITLE     A secreted PTEN phosphatase that enters cells to alter signaling and
            survival.
  JOURNAL   Science. 2013 Jul 26;341(6144):399-402. doi: 
            10.1126/science.1234907. Epub 2013 Jun 6.
  PUBMED    23744781
REFERENCE   2  (bases 1 to 1367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1234907"; journalName: "Science"; date: 
            "2013-07-26- 26"; volume: "341"; issue: "6144"; pages: "399-402"
COMMENT     Sequence Label: JpExpress404 PTEN
FEATURES             Location/Qualifiers
     source          1..1367
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1290..1331
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1341..1358
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aggaggtaaa acatatgacc gcgattatca aagagatcgt gtcccgtaac aagcgccgtt
       61 atcaagaaga tggctttgac ctggacctga cctacatcta cccgaacatt atcgcgatgg
      121 gttttccagc cgagcgcctg gagggcgtct accgtaataa catcgatgat gtggttcgtt
      181 ttctggacag caaacataag aaccattaca agatctataa tctgtgcgcc gagcgtcatt
      241 atgacaccgc gaaattcaat tgccgtgttg cgcagtaccc gttcgaggac cacaatccgc
      301 cgcaactgga actgattaaa ccgttctgcg aggacctgga tcagtggctg tccgaggacg
      361 acaaccacgt ggccgcgatt cactgtaagg ccggcaaagg tcgtaccggc gtcatgatct
      421 gcgcgtacct gctgcaccgc ggtaagtttc tgaaggcgca agaagcgctg gacttttacg
      481 gcgaggtgcg tacccgcgac aaaaagggtg tcaccatccc gagccaacgc cgctatgttt
      541 actactacag ctatttgctg aaaaaccacc tggattatcg tcctgtcgcc ctgttgtttc
      601 acaagatgat gtttgaaacg attccgatgt tcagcggcgg tacctgtaat ccgcagttcg
      661 tcgtttgtca gctgaaggtg aagatctaca gctctaatag cggtccgacc cgtcgcgaag
      721 ataagtttat gtatttcgaa ttcccgcagc cgctgccggt ttgtggtgac atcaaggtgg
      781 agttcttcca taaacaaaac aagatgttga agaaagataa gatgttccat ttttgggtca
      841 acactttctt tattccgggt ccggaggaga ctagcgagaa agttgagaac ggtagcctgt
      901 gcgatcaaga aatcgatagc atttgcagca tcgaacgtgc ggataatgac aaagaatatc
      961 tggtcttgac cctgacgaaa aacgacctgg acaaagcgaa caaagataaa gcgaatcgct
     1021 atttcagccc gaatttcaaa gttaagctgt actttaccaa aactgtggag gaaccgagca
     1081 atccggaggc gtctagctct accagcgtga ccccggacgt cagcgacaat gagccggacc
     1141 actatcgtta cagcgacacg accgacagcg atcctgaaaa tgaaccgttt gacgaagatc
     1201 aacatacgca gattaccaag gtgaaaggca acagcgcgga tattcagcat tctggtggcc
     1261 gtagcagctt ggaaggccct cgcttcgagg gtaagccgat tccgaatccg ctgctgggtc
     1321 tggatagcac gcgcacgggt caccaccacc atcaccacta actcgag
//
