LOCUS       sequence-83972-e         662 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, 
            PconNoHindM8:RBS(st3):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 662)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1947
FEATURES             Location/Qualifiers
     source          1..662
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(57..74)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      227..313
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      405..432
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gagctctaca ataaaagcta gcttggctgt tttggcggat gagagaagat tttcagcctg
       61 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt
      121 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat
      181 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa
      241 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct
      301 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg
      361 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac
      421 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata
      481 tgtatccgct caggatcctc gagcaccgtc gttgttgaca tttttatgct tggcggttat
      541 aatggattcc acacacccgg gtaaggagga aaatgtctag attagataaa agtaaagtga
      601 ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca acccgtaaac
      661 tc
//
