LOCUS       sequence-83973-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, 
            PconNoHind:RBS(st3):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1855
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(273..896)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFXLELIICGLEKQLKCESGS"
     primer_bind     804..823
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cttccttcgg ggcatttacg ccaatacgta cctggttgcc ctttacccct aaaactgtca
       61 cggtgacctc atccccaatc atgagggtct caccaactcg acgagtcaga atcagcattt
      121 tcctccttag cggccgcctg tgtggaatcc attataaccg ccaagcataa aaatgtcaac
      181 aacgacggtg ctcgagaatt cactgctcac aagaaaaaag gcacgtcatc tgacgtgcct
      241 tttttatttg tactaccctg gacgattaaa gcttaagacc cactttcaca tttaagttgt
      301 ttttctaatc cgcatatgat caattcaagg ncgaataaga aggctggctc tgcaccttgg
      361 tgatcaaata attcgatagc ttgtcgtaat aatggcggca tactatcagt agtaggtgtt
      421 tccctttctt ctttagcgac ttgatgctct tgatcttcca atacgcaacc taaagtaaaa
      481 tgccccacag cgctgagtgc atataatgca ttctctagtg aaaaaccttg ttggcataaa
      541 aaggctaatt gattttcgag agtttcatac tgtttttctg taggccgtgt acctaaatgt
      601 acttttgctc catcgcgatg acttagtaaa gcacatctaa aacttttagc gttattacgt
      661 aaaaaatctt gccagctttc cccttctaaa gggcaaaagt gagtatggtg cctatctaac
      721 atctcaatgg ctaaggcgtc gagcaaagcc cgcttatttt ttacatgcca atacaatgta
      781 ggctgctcta cacctagctt ctgggcgagt ttacgggttg ttaaaccttc gattccgacc
      841 tcattaagca gctctaatgc gctgttaatc actttacttt tatctaatct agacattttc
      901 ctccttaccc
//
