LOCUS       sequence-83976-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PconNoHindM12:glgC::gfp, PconNoHind:RBS(st3):tetR, 
            PconNoHindM8:RBS(st3):csrA .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1947
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(268..891)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFXFGXELIIXXXEXQLKCESGX"
     primer_bind     799..818
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cttccttcgg ggcatttacn ccnatacgta nctggttgcc ctttacccct aaaantgtca
       61 cggggacctc atccccnagt catgagggtc tcaccaactc gacgagtcnn aatcancant
      121 ttcctcctta gcggccgcga atccaccana acngccaagc ntaaaaatgt aaacaacgac
      181 ggtgctcgag aattnactgc tcacaagaaa aaaggcacgt catctgacgn gcctttttta
      241 tttgtactna ccctgnacga ttaaagctta nnacccactt tcacatttaa gttgtntttc
      301 nnntcnncat atgatcaatt cnnggccgaa nnagaaggct ggctctgcac cttggtgatc
      361 aaataattcg atagcttgtc gtaataatgg cggcatacta tcagtagtag gtgtttccct
      421 ttcttcttta gcgacttgat gctcttgatc ttccaatacg caacctaaag taaaatgccc
      481 cacagcgctg agtgcatata atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc
      541 taattgattt tcgagagttt catactgttt ttctgtaggc cgtgtaccta aatgtacttt
      601 tgctccatcg cgatgactta gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa
      661 atcttgccag ctttcccctt ctaaagggca aaagtgagta tggtgcctat ctaacatctc
      721 aatggctaag gcgtcgagca aagcccgctt attttttaca tgccaataca atgtaggctg
      781 ctctacacct agcttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt
      841 aagcagctct aatgcgctgt taatcacttt acttttatct aatctagaca ttttcctcct
      901 tacccggggt gtgtggaatc cattataacc gccaa
//
