LOCUS       sequence-83978-e         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetR
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1914
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(59..76)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      229..315
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      407..434
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atgagctcta caataaaagc tagcttggct gttttggcgg atgagagaag attttcagcc
       61 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
      121 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
      181 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
      241 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      301 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
      361 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
      421 acggatggcc tttttgcgtt tctacaaact cttttgttta tttttctaaa tacattcaaa
      481 tatgtatccg ctcaggatcc tcgagcaccg tcgttgttga catttttatg cttggcggtt
      541 ataatggatt ccacacaccc gggtaaggag gaaaatgtct agattagata aaagtaaagt
      601 gattaacagc gcattagagc tgcttaatga ggtcggaatc gaaggtttaa caacccggaa
      661 actcgcccag aagctaggtg tagagcagcc tacatt
//
