LOCUS       sequence-83981-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:pgaAleadFull::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1724
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    375..393
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    400..418
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(439..457)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(690..709)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct cagcatgctt tttctgctac
      121 ttgaatacat cctgtattac tccatgtatt gccaaaatct ctctctgtat ctaattacag
      181 gtaactgaaa agaaagatat ttttgcacct cataatccgt tattaaacgc ggaagagaga
      241 cgtgaattgt tgatgatgag aagaagaaat gatgagcaga gtgtccatat aaaatccttt
      301 tctcgcccga aaatccattc caatgatgag gatcttcagg aatacggcat aaatcccaat
      361 gcctgtcgac agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact
      421 cgaggtgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      481 taatggtttc ttagacgtcg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
      541 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
      601 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaaggt ggattgcacg
      661 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      721 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      781 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
      841 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
//
