LOCUS       sequence-83982-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1746
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    180..198
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(244..262)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(495..514)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgccctcgat ctcgaactcg tggccgttca cggagccctc catgtgcacc ttgaagcgca
       61 tgaactcctt gatgatggcc atgttatcct cctcgccctt gctcagcatg ctactaattc
      121 tcaaatagtc tttttccata aagctacacc aatctgtagg ttgtagatcg tcgacagtat
      181 ctctatcact gatagggatg tcaatctcta tcactgatag ggactcgagg tgaagacgaa
      241 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
      301 cgtcggaatt gccagctggg gcgccctctg gtaaggttgg gaagccctgc aaagtaaact
      361 ggatggcttt cttgccgcca aggatctgat ggcgcagggg atcaagatct gatcaagaga
      421 caggatgagg atcgtttcgc atgattgaac aaggtggatt gcacgcaggt tctccggccg
      481 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
      541 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
      601 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
      661 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
      721 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
      781 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
      841 accaccaagc gaaacatcgc atcga
//
