LOCUS       sequence-83984-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:glgClead::mCherry, PLlacO-1:csrB
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1790
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    195..213
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(259..277)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(510..529)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
       61 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca gcatgctaac
      121 catgactaac tcctttttta tcatctctgg aacacacaca atccgtgtgc aggtccctgc
      181 cagagtcgac agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggact
      241 cgaggtgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
      301 taatggtttc ttagacgtcg gaattgccag ctggggcgcc ctctggtaag gttgggaagc
      361 cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc aggggatcaa
      421 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaaggt ggattgcacg
      481 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      541 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      601 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt
      661 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
      721 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
      781 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
      841 ctacct
//
