LOCUS       sequence-83985-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1722
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    196..214
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    221..239
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(260..278)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(511..530)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc agcatgctaa
      121 ccatgactaa ctcctttttt atcatctctg gaacacacac aatccgtgtg caggtccctg
      181 ccagagtcga cagtatctct atcactgata gggatgtcaa tctctatcac tgatagggac
      241 tcgaggtgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      301 ataatggttt cttagacgtc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
      361 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
      421 agatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaagg tggattgcac
      481 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
      541 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
      601 gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc gcggctatcg
      661 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
      721 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
      781 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
      841 gctacctgcc cattcgacca ccaagcga
//
