LOCUS       sequence-83986-e        1225 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of human HRAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 1225)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-HRAS
FEATURES             Location/Qualifiers
     source          1..1225
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             168..737
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=H-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(749..773)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgaaggctg ccgaccccgg gggtggacca tcctctagac tgccggatct agctagttaa
       61 ttaaggatcc cagtgtggtg gtacgggaaa tcacaagttt gtacaaaaaa gcaggcttta
      121 aaggaaccaa ttcagtcgac tggatctcga gctcaagctt cgaattcatg acggaatata
      181 agcttgttgt tgttggcgcc ggcggtgtgg gcaagagtgc gctgaccatc cagctgatcc
      241 agaaccattt tgtggacgaa tacgacccca ctatagagga ttcctaccgg aagcaggtgg
      301 tcattgatgg ggagacgtgc ctgttggaca tcctggatac cgccggccag gaggagtaca
      361 gcgccatgcg ggaccagtac atgcgcaccg gggagggctt cctgtgtgtg tttgccatca
      421 acaacaccaa gtcttttgag gacatccacc agtacaggga gcagatcaaa cgggtgaagg
      481 actcggatga cgtgcccatg gtgctggtgg ggaacaagtg tgacctggct gcacgcactg
      541 tggaatctcg gcaggctcag gacctcgccc gaagctacgg catcccctac atcgagacct
      601 cggccaagac ccggcaggga gtggaggatg ccttctacac gttggtgcgt gagatccggc
      661 agcacaagct gcggaagctg aaccctcctg atgagagtgg ccccggctgc atgagctgca
      721 agtgtgtgct ctcctgagga tcatctagac ccagctttct tgtacaaagt ggtgatattc
      781 cagctgagcg ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg
      841 ggcaggccat gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaaactcg
      901 agcggccgcc agcacagtgg tcgacgataa aataaaagat tttatttagt ctccagaaaa
      961 aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt
     1021 gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg tcaggaacag
     1081 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct
     1141 cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca
     1201 gttcctgccc cggctcaggg ccaaa
//
