LOCUS       sequence-83987-e        1157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of human KRAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 1157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-KRAS
FEATURES             Location/Qualifiers
     source          1..1157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    93..117
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             138..704
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
     protein_bind    complement(721..745)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgaaggctg ccgaccccgg gggtggacca tcctctagac tgccggatct agctagttaa
       61 ttaaggatcc cagtgtggtg gtacgggaaa tcacaagttt gtacaaaaaa gcaggctccg
      121 cggccgcccc cttcaccatg actgaatata aacttgtggt agttggagct ggtggcgtag
      181 gcaagagtgc cttgacgata cagctaattc agaatcattt tgtggacgaa tatgatccaa
      241 caatagagga ttcctacagg aagcaagtag taattgatgg agaaacctgt ctcttggata
      301 ttctcgacac agcaggtcaa gaggagtaca gtgcaatgag ggaccagtac atgaggactg
      361 gggagggctt tctttgtgta tttgccataa ataatactaa atcatttgaa gatattcacc
      421 attatagaga acaaattaaa agagttaagg actctgaaga tgtacctatg gtcctagtag
      481 gaaataaatg tgatttgcct tctagaacag tagacacaaa acaggctcag gacttagcaa
      541 gaagttatgg aattcctttt attgaaacat cagcaaagac aagacagggt gttgatgatg
      601 ccttctatac attagttcga gaaattcgaa aacataaaga aaagatgagc aaagatggta
      661 aaaagaagaa aaagaagtca aagacaaagt gtgtaattat gtaaaagggt gggcgcgccg
      721 acccagcttt cttgtacaaa gtggtgatat tccagctgag cgccggtcgc taccattacc
      781 agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg
      841 taaggaaatc cattatgtac tatttaaact cgagcggccg ccagcacagt ggtcgacgat
      901 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
      961 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
     1021 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
     1081 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
     1141 atatgggcca aacagga
//
