LOCUS       sequence-83992-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1747
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    184..202
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    209..227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(248..266)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(499..518)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cctcgccctc gatctcgaac tcgtggccgt tcacggagcc ctccatgtgc accttgaagc
       61 gcatgaactc cttgatgatg gccatgttat cctcctcgcc cttgctcagc atgctttccg
      121 ttgtcttctt gatcattgcc actccttttt cacagttcct tgtgcgcgct attgtcgaca
      181 gtatctctat cactgatagg gatgtcaatc tctatcactg atagggactc gaggtgaaga
      241 cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct
      301 tagacgtcgg aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta
      361 aactggatgg ctttcttgcc gccaaggatc tgatggcgca ggggatcaag atctgatcaa
      421 gagacaggat gaggatcgtt tcgcatgatt gaacaaggtg gattgcacgc aggttctccg
      481 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
      541 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
      601 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
      661 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
      721 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
      781 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
      841 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
//
