LOCUS       sequence-83993-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PconNoHindM2:glgC::gfp, PconNoHind:RBS(st3):tetR
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1757
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(28..651)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     559..578
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     terminator      complement(792..819)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 atttgtacta ccctgtacga ttaaagctta agacccactt tcacatttaa gttgtttttc
       61 taatccgcat atgatcaatt caaggccgaa taagaaggct ggctctgcac cttggtgatc
      121 aaataattcg atagcttgtc gtaataatgg cggcatacta tcagtagtag gtgtttccct
      181 ttcttcttta gcgacttgat gctcttgatc ttccaatacg caacctaaag taaaatgccc
      241 cacagcgctg agtgcatata atgcattctc tagtgaaaaa ccttgttggc ataaaaaggc
      301 taattgattt tcgagagttt catactgttt ttctgtaggc cgtgtaccta aatgtacttt
      361 tgctccatcg cgatgactta gtaaagcaca tctaaaactt ttagcgttat tacgtaaaaa
      421 atcttgccag ctttcccctt ctaaagggca aaagtgagta tggtgcctat ctaacatctc
      481 aatggctaag gcgtcgagca aagcccgctt attttttaca tgccaataca atgtaggctg
      541 ctctacacct agcttctggg cgagtttacg ggttgttaaa ccttcgattc cgacctcatt
      601 aagcagctct aatgcgctgt taatcacttt acttttatct aatctagaca ttttcctcct
      661 tacccgggtg tgtggaatcc attataaccg ccaagcataa aaatgtcaac aacgacggtg
      721 ctcgaggatc ctgagcggat acatatttga atgtatttag aaaaataaac aaaagagttt
      781 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttaatttga tgcctggcag
      841 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttc
//
