LOCUS       sequence-83997-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PconNoHind:csrB, PLtetO-1:RBS(st3):csrD
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL1575
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      622..708
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    728..746
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    753..771
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 ctgcgtgcat ccaccttgtt caatcatgcg aaacgatcct catcctgtct cttgatcaga
       61 tcttgatccc ctgcgccatc agatccttgg cggcaagaaa gccatccagt ttactttgca
      121 gggcttccca accttaccag agggcgcccc agctggcaat tccgacgtct cgagcaccgt
      181 cgttgttgac atttttatgc ttggcggtta taatggattc cacacaggtc gacgagtcag
      241 acaacgaagt gaacatcagg atgatgacac ttctgcagga cacaccagga tggtgtttca
      301 gggaaaggct tctggatgaa gcgaagagga tgacgcagga cgcgttaaag gacacctcca
      361 ggatggagaa tgagaaccgg tcaggatgat tcggtgggtc aggaaggcca gggacacttc
      421 aggatgaagt atcacatcgg ggtggtgtga gcaggaagca atagttcagg atgaacgatt
      481 ggccgcaagg ccagaggaaa agttgtcaag gatgagcagg gagcaacaaa agtagctgga
      541 atgctgcgaa acgaaccggg agcgctgtga atacagtgct cccttttttt attgggccca
      601 tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      661 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag
      721 acctaggtcc ctatcagtga tagagattga catccctatc agtgatagag atactgcggc
      781 cgctaaggag gaaaatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg
      841 ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac gcca
//
