LOCUS       sequence-83998-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + fimS (off orientation)::gfp::Asp terminator + CamR + ColE1
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 246
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(104..151)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(168..827)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     742..761
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caataagctt taatcgtaca gggtagtaca aataaaaaag gcacgtcaga tgacgtgcct
       61 tttttcttgt gagcagtgaa ttcgtgtagg ctggagctgc ttcgaagttc ctatactttc
      121 tagagaatag gaacttcgga ataggaactt catttaaatg gcgcgcctta cgccccgccc
      181 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
      241 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
      301 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
      361 aaactggtga aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct
      421 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
      481 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
      541 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
      601 gccatacgta attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
      661 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
      721 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
      781 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
      841 gctcctgaaa atctcgacaa ctcaaaa
//
