LOCUS       sequence-84000-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:ycdTlead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1746
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      272..358
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    413..429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(750..767)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccccgaggag gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
       61 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      121 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      181 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      241 taagggccca tggtacgcgt gctagaggca tcaaataaaa cgaaaggctc agtcgaaaga
      301 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc
      361 gccgccctag acctaggcgc ggatccataa atgtgagcgg ataacattga cattgtgagc
      421 ggataacaag atactcccgg gtaaggagga aaaaaaatgc tgattctgac tcgtcgagtt
      481 ggtgagaccc tcatgattgg ggatgaggtc accgtgacag ttttaggggt aaagggcaac
      541 caggtacgta ttggcgtaaa tgccccgaag gaagtttctg ttcaccgtga agagatctac
      601 cagcgtatcc aggctgaaaa atcccagcag tccagttact aaggtacctg aaagaagctt
      661 taatcgtaca gggtagtaca aataaaaaag gcacgtcaga tgacgtgcct tttttcttgt
      721 gagcagtgaa ttcggatata ttccgcttcc tcgctcactg actcgctacg ctcggtcg
//
