LOCUS       sequence-84001-e         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:RBS(st3):csrA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1590
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    147..165
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    172..190
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(211..229)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(462..481)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 acagaaactt ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa
       61 actgtcacgg tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc
      121 agcattttcc tccttacccg ggagtatctc tatcactgat agggatgtca atctctatca
      181 ctgataggga ctcgaggtga agacgaaagg gcctcgtgat acgcctattt ttataggtta
      241 atgtcatgat aataatggtt tcttagacgt cggaattgcc agctggggcg ccctctggta
      301 aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc
      361 gcaggggatc aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag
      421 gtggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
      481 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
      541 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag
      601 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
      661 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
      721 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
      781 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgca
//
