LOCUS       sequence-84005-e         512 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:glgClead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 512)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 512)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1722
FEATURES             Location/Qualifiers
     source          1..512
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    149..165
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(220..306)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 aaacttcctt cggggcattt acgccaatac gtacctggtt gccctttacc cctaaaactg
       61 tcacggtgac ctcatcccca atcatgaggg tctcaccaac tcgacgagtc agaatcagca
      121 ttttttttcc tccttacccg ggagtatctt gttatccgct cacaatgtca atgttatccg
      181 ctcacattta tggatccgcg cctaggtcta gggcggcgga tttgtcctac tcaggagagc
      241 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
      301 tatttgatgc ctctagcacg cgtaccatgg gcccttactt gtacagctcg tccatgccgc
      361 cggtggagtg gcggccctcg gcgcgttcgt actgttccac gatggtgtag tcctcgttgt
      421 gggaggtgat gtccaacttg atgttgacgt tgtaggcgcc gggcagctgc acgggcttct
      481 tggccttgta ggtggtcttg acctcagcgt cg
//
