LOCUS       sequence-84006-e         797 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PLtetO-1:ydeHlead::mCherry, PLlacO-1:RBS(st7):csrA
             .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 797)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL1747
FEATURES             Location/Qualifiers
     source          1..797
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      273..359
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    414..430
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(751..768)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
       61 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
      121 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
      181 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
      241 gtaagggccc atggtacgcg tgctagaggc atcaaataaa acgaaaggct cagtcgaaag
      301 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
      361 cgccgcccta gacctaggcg cggatccata aatgtgagcg gataacattg acattgtgag
      421 cggataacaa gatactcccg ggtaaggagg aaaaaaaatg ctgattctga ctcgtcgagt
      481 tggtgagacc ctcatgattg gggatgaggt caccgtgaca gttttagggg taaagggcaa
      541 ccaggtacgt attggcgtaa atgccccgaa ggaagtttct gttcaccgtg aagagatcta
      601 ccagcgtatc caggctgaaa aatcccagca gtccagttac taaggtacct gaaagaagct
      661 ttaatcgtac agggtagtac aaataaaaaa ggcacgtcag atgacgtgcc ttttttcttg
      721 tgagcagtga attcggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt
      781 tcgactgcgg cgagcgg
//
