LOCUS       sequence-84008-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PLtetO-1::RBS (st3) fimB::Asp terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL 1977
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    650..668
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    675..693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(714..732)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        800..904
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtactaccct gtacgattaa agcttctata aaacagcgtg acgctgtcgt cctctggctc
       61 tatcccagat gccgtaaaaa cgccctgcat tgctggcggt ataccagaca gtatgacgaa
      121 tattgcgatg cccaagataa tcctggataa gtcgcgtatc tattcccata ttcgccaaag
      181 caaaaccaca cgaatggcgt aacatgtgcg gatgaatctc cagtgacaac ccggcattac
      241 caccggaagt cgagataata tggtaaaact gttgccgaga aagcggattc cccttacgtg
      301 ataaaaatac ccactcgctc tcagcatgcg ggtacgaagt acggatactc aaccagtttt
      361 ttaaagcctg aacttcttta ttcaatagcg ggtgcgttgt tgaaaagcct ttttttaatc
      421 gatggatata tatacacttt gccttaagat caatatccga aatcctcaat cgacaaattt
      481 cactcgcccg gaaaccatga ataaaacaaa gcaaagtcag acaataatta cgtgctgcat
      541 gaggcccggt atttgctgct ttaaggagtg attcgatttc actatgggtc aggaagttcc
      601 tttttttgtt atcagcctta ttcttcattt tcctccttac ccgggagtat ctctatcact
      661 gatagggatg tcaatctcta tcactgatag ggactcgagg tgaagacgaa agggcctcgt
      721 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
      781 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
      841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      901 gagtatgagt attcaacatt tc
//
