LOCUS       sequence-84016-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS 
            (st7) gfp::Asp terminator + ColE1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1278
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    141..159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(205..223)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        291..395
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(614..633)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatttttt
      121 ttcctcctta gtcgacagta tctctatcac tgatagggat gtcaatctct atcactgata
      181 gggactcgag gtgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      241 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      301 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      361 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      421 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      481 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      541 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      601 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
      661 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
      721 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
      781 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
      841 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
      901 aagccatacc aaacgacgag cgtgac
//
