LOCUS       sequence-84019-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) 
            mCherry::Asp terminator + ColE1 .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1277
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    79..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(150..236)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(308..328)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca ttttttttcc
       61 tccttacccg ggagtatctt gttatccgct cacaatgtca atgttatccg ctcacattta
      121 tggatccgcg cctaggtcta gggcggcgga tttgtcctac tcaggagagc gttcaccgac
      181 aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc
      241 ctctagcacg cgtaccatgg gcccttattt gtatagttca tccatgccat gtgtaatccc
      301 agcagctgtt acaaactcaa gaaggaccat gtggtctctc ttttcgttgg gatctttcga
      361 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
      421 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtgtct
      481 aattttgaag ttaactttga ttccattctt ttgtttgtct gccatgatgt atacattgtg
      541 tgagttatag ttgtattcca atttgtgtcc aagaatgttt ccatcttctt taaaatcaat
      601 accttttaac tcgattctat taacaaaggg tatcaccttc aaaacttgac ttcagcacgt
      661 gtctttgtag ttcccgtcat
//
