LOCUS       sequence-84021-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pLtetO-1:RhyB, pCon:T7(st3) (133bps)::mCherry, pLlacO-1:sodB::gfp .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: pHL 1394
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    555..573
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    580..598
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(665..692)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccctcgccct cgatctcgaa ctcgtggccg ttcacggagc cctccatgtg caccttgaag
       61 cgcatgaact ccttgatgat ggccatgtta tcctcctcgc ccttgctcac catttttttt
      121 cctccttagc atgccttagt aagactcatg ctcaagggcc aactgttcgc gagctaaacg
      181 ctcaccgtaa tggtcagcca gagtgttgaa cgggatagca gccagttcga tgtcagagaa
      241 gtcgttctta gcgatgttaa tcgtgttcat tttcctcctt agtcgacctg tgtggaatcc
      301 attataaccg ccaagcataa aaatgtcaac aacgacggtg ctcgagaatt cactgctcac
      361 aagaaaaaag gcacgtcatc tgacgtgcct tttttatttg tactaccctg tacgattaaa
      421 gcttgtggat aaattgagaa cgaaagatca aaaaaaaagc cagcacccgg ctggctaagt
      481 aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga
      541 tcgccccggg agtatctcta tcactgatag ggatgtcaat ctctatcact gatagggagg
      601 atcctgagcg gatacatatt tgaatgtatt tagaaaaata aacaaaagag tttgtagaaa
      661 cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg
      721 cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg
      781 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac
//
