LOCUS       sequence-84025-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + PLtetO-1::RBS (st7) fimB::Asp terminator + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1916
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    642..660
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    667..685
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(706..724)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 acgattaaag cttctataaa acagcgtgac gctgtcgtcc tctggctcta tcccagatgc
       61 cgtaaaaacg ccctgcattg ctggcggtat accagacagt atgacgaata ttgcgatgcc
      121 caagataatc ctggataagt cgcgtatcta ttcccatatt cgccaaagca aaaccacacg
      181 aatggcgtaa catgtgcgga tgaatctcca gtgacaaccc ggcattacca ccggaagtcg
      241 agataatatg gtaaaactgt tgccgagaaa gcggattccc cttacgtgat aaaaataccc
      301 actcgctctc agcatgcggg tacgaagtac ggatactcaa ccagtttttt aaagcctgaa
      361 cttctttatt caatagcggg tgcgttgttg aaaagccttt ttttaatcga tggatatata
      421 tacactttgc cttaagatca atatccgaaa tcctcaatcg acaaatttca ctcgcccgga
      481 aaccatgaat aaaacaaagc aaagtcagac aataattacg tgctgcatga ggcccggtat
      541 ttgctgcttt aaggagtgat tcgatttcac tatgggtcag gaagttcctt tttttgttat
      601 cagccttatt cttcattttt tttcctcctt acccgggagt atctctatca ctgataggga
      661 tgtcaatctc tatcactgat agggactcga ggtgaagacg aaagggcctc gtgatacgcc
      721 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
      781 ggggaaatgt gcgcggaacc cctatttgtt tattttttct aaatacattc aaatatgtat
      841 ccgctcatga gacaataacc
//
