LOCUS       sequence-84033-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + fimS::gfpAAV::Asp terminator + CamR + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Hung M, Chang E, Hussein R, Frazier K, Shin JE, Sagawa S, Lim HN
  TITLE     Modulating the frequency and bias of stochastic switching to control
            phenotypic variation.
  JOURNAL   Nat Commun. 2014 Aug 4;5:4574. doi: 10.1038/ncomms5574.
  PUBMED    25087841
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2014-08-4"; volume: "5:4574. doi"; pages: " 
            10.1038/ncomms5574"
COMMENT     Sequence Label: pHL 1903
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(140..187)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     778..797
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 caagcagcaa acgacgaaaa ctacgctgca gcagtttaat aagcttaaat cgtacagggt
       61 agtacaaata aaaaaggcac gtcagatgac gtgccttttt tcttgtgagc agtgaattcg
      121 tgtaggctgg agctgcttcg aagttcctat actttctaga gaataggaac ttcggaatag
      181 gaacttcatt taaatggcgc gccttacgcc ccgccctgcc actcatcgca gtactgttgt
      241 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
      301 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
      361 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
      421 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
      481 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
      541 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
      601 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgtaattc cggatgagca
      661 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
      721 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
      781 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aaccggtggt
      841 atatccagtg attttttt
//
