LOCUS       sequence-84035-e         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AmpR + fimS::gfpLVA::Asp terminator + CamR + ColE1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     Lim Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pHL 1904
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(147..194)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     785..804
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gactatacaa gcagcaaacg acgaaaacta cgctttagta gcttaataag ctttaatcgt
       61 acagggtagt acaaataaaa aaggcacgtc agatgacgtg ccttttttct tgtgagcagt
      121 gaattcgtgt aggctggagc tgcttcgaag ttcctatact ttctagagaa taggaacttc
      181 ggaataggaa cttcatttaa atggcgcgcc ttacgccccg ccctgccact catcgcagta
      241 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
      301 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
      361 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
      421 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
      481 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
      541 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
      601 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac gtaattccgg
      661 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
      721 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
      781 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
      841 ggtggtatat ccagtgat
//
