LOCUS       sequence-84041-e        1169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression of human LIN-41 / TRIM71 containing only the 
            NHL repeats and 3 N-terminal HA tags .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Worringer KA, Rand TA, Hayashi Y, Sami S, Takahashi K, Tanabe K, 
            Narita M, Srivastava D, Yamanaka S
  TITLE     The let-7/LIN-41 pathway regulates reprogramming to human induced 
            pluripotent stem cells by controlling expression of 
            prodifferentiation genes.
  JOURNAL   Cell Stem Cell. 2014 Jan 2;14(1):40-52. doi: 
            10.1016/j.stem.2013.11.001. Epub 2013 Nov 14.
  PUBMED    24239284
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.11.001"; journalName: "Cell Stem Cell"; date: 
            "2014-01-2- 2"; volume: "14"; issue: "1"; pages: "40-52"
COMMENT     Sequence Label: pMXs-hs-3xHA-NHL only-LIN-41
FEATURES             Location/Qualifiers
     source          1..1169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..24
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    50..424
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     362..378
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      481..490
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             493..582
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
ORIGIN
        1 atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc catatgagat
       61 cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga caagagttac
      121 taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc acgaagtctg
      181 gagacctctg gcggcagcct accaagaaca actggaccga ccggtggtac ctcaccctta
      241 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg
      301 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc
      361 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag
      421 actgccggat ctagctagtt aattaaggat cccagtgtgg tggtacggga attcctgcag
      481 gccaccatgg catacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg
      541 gactatgcag gatcctatcc atatgacgtt ccagattacg ctatgggccg cagctacgtg
      601 ggcattgggc tcccgggcct gagcttcggc agtgagggtg acagcgatgg caagctctgc
      661 cgcccttggg gtgtgagtgt agacaaggag ggctacatca ttgtcgccga ccgcagcaac
      721 aaccgcatcc aggtgttcaa gccctgcggc gccttccacc acaaattcgg caccctgggc
      781 tcccggcctg ggcagttcga ccgaccagcc ggcgtggcct gtgacgcctc acgcaggatc
      841 gtggtggctg acaaggacaa tcatcgcatc cagatcttca cgttcgaggg ccagttcctc
      901 ctcaagtttg gtgagaaagg aaccaagaat gggcagttca actacccttg ggatgtggcg
      961 gtgaattctg agggcaagat cctggtctca gacacgagga accaccggat ccagctgttt
     1021 gggcctgatg gtgtcttcct aaacaagtat ggcttcgagg gggctctctg gaagcacttt
     1081 gactccccac ggggtgtggc cttcaaccat gagggccact tggtggtcac tgacttcaac
     1141 aaccaccggc tcctggttat tcaccccga
//
